Dioxygenase mutants and use thereof in synthesizing (2S,3R)-3-hydroxypipecolic acid
US12415992B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 17, 2025 |
| Grant date | Sep 16, 2025 |
| Priority date | — |
| Expiry date | Jan 17, 2045 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y114/11028
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
By mutating arginine at position 97 and asparagine at position 43 of a wild type L-proline-3-hydroxylase, a mutated L-proline-3-hydroxylase is obtained. The mutated L-proline-3-hydroxylase comprises a variant of the wild-type L-proline-3-hydroxylase having SEQ ID NO:1, which comprises a substitution of the arginine corresponding to position 97 (R97) of SEQ ID NO:1 and a substitution of the asparagine corresponding to position 43 (N43) of SEQ ID NO:1; wherein the amino acid substituted into the mutated L-proline-3-hydroxylase at position R97 is methionine, and wherein the amino acid substituted into the mutated L-proline-3-hydroxylase at position N43 is selected from the group consisting of threonine (T), alanine (A), valine (V), serine(S), aspartic acid (D), and lysine (K).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.