Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring
US12416000B2 · kind B2 · utility
0Cited by
10References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 27, 2021 |
| Grant date | Sep 16, 2025 |
| Priority date | — |
| Expiry date | Feb 16, 2044 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2523/307
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and apparatuses to non-destructively and periodically sample a small quantity of intracellular proteins and mRNA from the same single cell or cells for an extended period of time. Specifically, describe herein are non-perturbative methods for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and nucleic acids from a variety of cell types using systems including nanostraws.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.