Preparation method and application of single-chain antibody fragment targeting SARS-CoV-2 nucleocapsid protein
US12421300B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 19, 2024 |
| Grant date | Sep 23, 2025 |
| Priority date | — |
| Expiry date | Nov 19, 2044 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/165
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are a preparation method and application of a single-chain antibody fragment targeting SARS-CoV-2 nucleocapsid protein, aiming to provide a single-chain antibody fragment with high affinity and strong specificity. The present disclosure obtains three single-chain antibody fragments targeting SARS-CoV-2 nucleocapsid protein through construction of a nanobody phage library and phage screening technology. The single-chain antibody fragment consists of a heavy chain variable region, a 15aa connecting peptide, and a light chain variable region connected in sequence, and has an amino acid sequence shown in SEQ ID NO: 1-SEQ ID NO: 3. The single-chain antibody fragment in the present disclosure has excellent binding performance and specificity with the SARS-CoV-2 nucleocapsid protein, can be applied to a variety of immunoassay platforms of the SARS-CoV-2, and has broad application prospects in the field of SARS-CoV-2 detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.