Plant colonization assays using natural microbial barcodes
US12421519B2 · kind B2 · utility
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58References
18Claims
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Key dates
| Filing date | Jan 7, 2020 |
| Grant date | Sep 23, 2025 |
| Priority date | — |
| Expiry date | Nov 11, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure is drawn to methods of utilizing nucleic acid barcodes and corresponding amplifying sites in cells in which the barcodes naturally occur. These barcodes and amplifying sites are reconfigured into a single nucleic acid cassette that provides for ease of use in tagging particular species, strains, or variants of cells, each with a different barcode. These barcodes can be used to track the colonization capabilities of the barcoded cells. The present disclosure further provides for assays that utilize natural barcodes to measure relative microbial colonization ability of a plant root system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.