Method and kit for genotyping of multi-system red blood cell (RBC) blood group based on next-generation sequencing (NGS)
US12421549B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 9, 2025 |
| Grant date | Sep 23, 2025 |
| Priority date | — |
| Expiry date | Jan 9, 2045 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and a kit for genotyping of multi-system red blood cell (RBC) blood group based on next-generation sequencing (NGS) are provided. Multiplex PCR capture primer sets and amplification reaction conditions thereof can be designed using Illumina as a platform to simultaneously detect full-length coding regions and spliced flanking regions of 25 blood group genes in 17 RBC blood group systems among three reactions, thereby enabling rapid genotyping of the 17 RBC blood group systems and multiple key rare blood groups therein. The method and the kit are based on high-throughput sequencing and have a high detection efficiency and a low average cost; the method and the kit also break through a limitation of existing blood group gene detection technology that cannot distinguish haplotypes; moreover, variations including known mutation sites and unknown mutation sites can be detected to obtain comprehensive and accurate detection results.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.