Amino-substituted chromenoquinoline-based fluorescent marker, and preparation and use thereof
US12422443B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 30, 2021 |
| Grant date | Sep 23, 2025 |
| Priority date | — |
| Expiry date | Dec 30, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides an amino-substituted chromenoquinoline-based fluorescent marker, and preparation and use thereof. The amino group of an aromatic amine compound is subjected to a one-step cyclization reaction with two o-chloroformylcumarins, to obtain a series of nitrogen-containing heterocyclic compound. Such compounds have excellent fluorescence emission performance, and also have good photostability in various organic solvents. The problem of photostability of cyanine dyes is effectively solved, and these dyes are useful in marking various organelles at cellular level. The introduction of the nitrogen-containing heterocyclic ring improves the biocompatibility of the compound, thus laying a good foundation for use in organisms. Further, the raw materials used in such reactions are cheap and readily available, the steps of synthesizing the marker are simple and easy to operate, and the production cost is effectively reduced while not lowering the performance of the marker. Therefore, the present invention is of commercial value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.