RPA-PAND based enterovirus typing detection kit and detection method

US12428687B2 · kind B2 · utility

Assignee

• Hubei University · CN

Inventors

• Yanming Dong · 红钢城街道, CN
• Lixin Ma · 红钢城街道, CN
• Xuan Yang · 红钢城街道, CN
• Yuan Wang · Beijing, CN
• Yibin Cheng · 红钢城街道, CN
• Junbo Cao · 红钢城街道, CN

Key dates

• Filing date: Nov 27, 2024
• Grant date: Sep 30, 2025
• Expiry date: Nov 27, 2044

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a recombinase polymerase amplification (RPA)-PAND based enterovirus virus typing detection kit and a rapid sensitive detection method, where the detection kit includes a PfAgo protein three gDNAs(g1/g2/g3), MB and its corresponding gDNA(gMB) combination for specifically detection of enteroviruses EV71, CVA6, CVA10, CVA16 and the universal enterovirus (EVU). RPA-HFMD-PAND rapid nucleic acid detection methods for typing detection of common enterovirus type A pathogens EV71, CVA6, CVA10, CVA16 and enterovirus universal EVU are successfully established based on PfAgo protein mediated target detection in combination with an RPA technology. The method has the advantages of high sensitivity, high specificity, low cost, relatively low position targeting restriction, simple reaction system and no aerosol pollution, and can conduct multi-channel nucleic acid detection. The present disclosure provides a new reagent and approach for clinic diagnosis and epidemiology monitoring of a hand-foot-mouth disease (HFMD).