Sequential digestion of polypeptides for mass spectrometric analysis
US12429405B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 13, 2019 |
| Grant date | Sep 30, 2025 |
| Priority date | — |
| Expiry date | Jun 4, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/067
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The disclosed methods are directed to preparing polypeptides for multi-attribute analysis. The polypeptides are optionally denatured, reduced, and/or alkylated before being subjected to a first digestion. Following the first digestion the large and small fragments resulting from the digestion are separated from each other. A second digestion is then performed on the larger of the fragments. All of the fragments from the two digestions are then analyzed chromatographically, electrophoretically, or spectrometrically, or a combination of these methods. The methods are especially useful for the preparation of therapeutic polypeptides for analysis, especially those that are not easily cleaved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.