Yeasts
US3930946A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 26, 1973 |
| Grant date | Jan 6, 1976 |
| Priority date | — |
| Expiry date | Nov 26, 1993 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/923
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the preparation of a stable diploid of Candida lipolytica which comprises (a) in a first stage, separately cultivating two haploid strains of Candida lipolytica of opposed sign in a medium rich in assimilable carbon nutrient; (b) in a second stage, cultivating the two resultant strains together in a medium poor in assimilable carbon nutrient; and (c) in a third stage, after the appearance of diploid colonies, treating them with a mutagenic agent to stabilize them. The invention also comprises a process for the preparation of .alpha.-ketoglutaric acid which comprises cultivating a diploid strain obtained by crossing two Candida lipolytica haploid strains of opposite sign in a medium containing, as source of assimilable carbon, at least one hydrocarbon.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.