Process for isolating a fibrin-stabilizing factor

US3931399A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Hans Bohn · Marburg, DE
• Fritz Emmerich

Key dates

• Filing date: Dec 21, 1973
• Grant date: Jan 6, 1976
• Expiry date: Dec 21, 1993

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/851
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A process for isolating a fibrin-stabilizing factor, factor XIII, by extracting human placentae with sodium chloride solution; adding diamino-ethoxy-acridine lactate (DEAL); recovering the resultant precipitate and dissolving it in dilute alkali metal chloride solution containing a complexing agent; adding a quaternary ammonium base to the solution to precipitate inactive materials; reprecipitating the active principal from solution with DEAL; again dissolving the precipitate in dilute alkali metal chloride solution containing a complexing agent; reprecipitating the active principal by adding solid ammonium sulfate; forming a paste between the precipitate and a dilute solution of a complexing agent; dialyzing the paste; gel filtering the dialyzed product; precipitating the active principal from the active fractions with ammonium sulfate; dissolving the precipitate in a neutral tris-(hydroxymethyl)-aminomethane hydrochoric acid buffer containing a complexing agent; dialyzing the solution; and precipitating the factor XIII from the remaining solution in the form of euglobulin.