Purification of coenzyme A
US3935072A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1973 |
| Grant date | Jan 27, 1976 |
| Priority date | — |
| Expiry date | Jul 31, 1993 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Coenzyme A is purified by a process involving contacting a cyanogen halide-activated water-insoluble polysaccharide with a cell-free extract of a coenzyme A-producing microorganism to immobilize proteins such as enzymes having affinity for coenzyme A on the polysaccharide. The immobilized proteins are then contacted with a coenzyme A-containing solution to absorb coenzyme A followed by eluting coenzyme A from the immobilized proteins. Alternatively, the proteins having affinity for coenzyme A may be isolated from the cell-free extract prior to immobilization by absorbing the proteins on coenzyme A immobilized on a cyanogen halide-activated water-insoluble polysaccharide followed by eluting the proteins from the immobilized coenzyme A.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.