Fluorescent immunoassay employing total reflection for activation
US3939350A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 29, 1974 |
| Grant date | Feb 17, 1976 |
| Priority date | — |
| Expiry date | Apr 29, 1994 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/807
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Receptors are employed to which one or more fluroescent molecules are bound. When the receptors are bound to the epitopic sites on the surface and light of predetermined wave length is directed toward the surface, the fluorescing molecules are activated and fluoresce. In carrying out an assay, receptor is combined with an unknown suspected of containing molecules having the same epitopic sites bound to the surface. The receptor will bind to these molecules reducing the number of receptor sites available for binding to the epitopic sites on the surface. When the assay medium is contacted with the surface, the amount of receptor which binds to the surface, will be a function of available binding sites and, therefore, to the number of the molecules present in the unknown. Upon irradiation of the surface, substantially only the fluoroescent molecules bound to the surface will fluoresce. By monitoring the fluorescence, one can determine the presence and number of molecules of interest present in the unknown. The apparatus consists of a transparent solid sheet, conveniently as part of or optically connected to a prism, a light source set at an angle to provide total internal reflection a…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.