Purification of d-aminoacid oxidase
US3941657A · kind A · utility
1Cited by
2References
4Claims
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Key dates
| Filing date | Apr 16, 1975 |
| Grant date | Mar 2, 1976 |
| Priority date | — |
| Expiry date | Apr 16, 1995 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/814
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Process for the separation and purification of d-aminoacid oxidase by affinity chromatography. The process comprises contacting a mixture of proteins containing the d-aminoacid oxidase with a mineral carrier carrying haloalkylsilane grafts, upon which are fixed -- NH -- (CH.sub.2).sub.3 -- NH -- (CH.sub.2).sub.2 C.sub.6 H.sub.4 COOH residues. The enzyme retained by the carrier is then separated by a solution of ethyleneglycol. Use of the pure d-aminoacid oxidase for the preparation of cetonic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.