Coherent optical analyzer
US3947123A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 13, 1974 |
| Grant date | Mar 30, 1976 |
| Priority date | — |
| Expiry date | May 13, 1994 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S359/90
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Cells of a given type spread among an ensemble of cells of various types are identified and, if desired, counted. Coherent light is directed toward a monolayer of cells of various types. The light scattered by the monolayer of cells is collected by a lens and the collected light is filtered by an optimum filter located in the focal plane of the lens. The optimum filter is a minimum mean-square error linear filter which suppresses light scattered by cells other than the given type of cell whereby the light scattered by the given type is enhanced. The intensity of the light passed by the optimum filter is related to the number of cells of the given type contained in the monolayer of cells. If desired, a second lens may be used to collect the filtered light and focus it toward an integrating and squaring detector. The optimum filter may be obtained photographically. In one form, a first photographic negative of a monolayer of cells having a high percentage of cells of the given type is made at the focal plane of a transforming lens mounted so as to collect the light scattered by the monolayer; and, developed for a gamma of unity. Thereafter, a second photographic negative is made at t…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.