Steroid-delta-isomerase

US3948728A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Peter Roeschlau · Seeshaupt, DE
• Gunter Lang · Wernau, DE
• Klaus Beaucamp · Tutzing, DE
• Erich Bernt · Munich, DE

Key dates

• Filing date: Apr 9, 1975
• Grant date: Apr 6, 1976
• Expiry date: Apr 9, 1995

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/872
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The new enzyme steroid-.DELTA.-isomerase is provided, as well as a process for producing same, comprising digesting a micro-organism containing said isomerase, extracting same by destruction of the cell wall with a buffer solution containing a non-ionic surfactant, centrifuging the resulting mass and discarding the precipitate, applying the extract to an anion exchanger, eluting the enzyme with a buffer solution containing a non-ionic surfactant, heating the eluate, separating the precipitate and isolating the enzyme therefrom from the supernatant.