Process for the purification of synthetic calcitonins
US3948876A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 3, 1974 |
| Grant date | Apr 6, 1976 |
| Priority date | — |
| Expiry date | Dec 3, 1994 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S220/902
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Peptides of the calcitonin type when obtained by synthesis can be purified in a simple manner by forming inner salts, which precipitate from aqueous solutions of salts of the peptides with acids or bases when these solutions are adjusted to the isoelectric range. Impurities remain in solution, while the inner salts separate practically quantitatively. The precipitation is completed after several hours. The peptides can then be recuperated by treatment with an acid, especially a therapeutically suitable acid. The process affords products which are superior to those obtained by purification methods hitherto known and gives higher yields. Other long-chain peptides such as ACTH, insulin or glucagon cannot be purified in this manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.