Purification of dehydrogenases

US3951744A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Albert Roder · Seeshaupt, DE
• Klaus Beaucamp · Tutzing, DE
• Gunter Weimann
• Walter Schneider · Schriesheim, DE
• Klaus Muhlegger · Polling, DE

Key dates

• Filing date: Jul 16, 1974
• Grant date: Apr 20, 1976
• Expiry date: Jul 16, 1994

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/815
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

NADP-dependent dehydrogenases are separated and purified by subjecting mixtures containing it to affinity chromatography using as the affinity chromatography material a nucleotide with a phosphate group in the 2'- or 3'-position, bound to a solid carrier.