Method and erythrocyte preparation for reverse blood grouping
US3956477A · kind A · utility
9Cited by
3References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 8, 1973 |
| Grant date | May 11, 1976 |
| Priority date | — |
| Expiry date | Jun 8, 1993 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/106664
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein is a stable preparation of human erythrocytes for use in reverse blood typing comprising erythrocytes hemolyzed by gradual hypotonic hemolysis using a sequence of aqueous hypotonic buffers to form stroma wherein the stroma cells are in the form of discoid bodies having the reticular membrane of the cell substantially intact. The stroma reagent has been found to be highly stable and antigenic in the sense that it contains blood group antigens (carried on the stroma cells), and can be admixed with human blood serum or plasma to determine the presence of hemologous blood group antibodies.
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