Preparation of an enzyme-cellulose complex
US3960666A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 8, 1974 |
| Grant date | Jun 1, 1976 |
| Priority date | — |
| Expiry date | Oct 8, 1994 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/12
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Enzymes are fixed to cellulose containing 5 to 25% lignin to form an enzyme-cellulose complex by a process involving treating cellulose pieces with an alkaline solution to remove lignin on the surface of the pieces, treating the resultant cellulose pieces with a sulfochlorinating or a chlorinating agent in an excess of pyridine until sulfur and/or chlorine is combined with the cellulose, washing the sulfur and/or chlorine-containing cellulose pieces and treating the pieces with an aqueous enzyme solution to attach enzymes thereto. A very important application is preparation of a rennet-cellulose complex and using the complex for coagulating milk in making cheese.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.