Lyophilized human hemoglobin standard for colorimetric determination of total hemoglobin
US3964865A · kind A · utility
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Key dates
| Filing date | Jan 30, 1975 |
| Grant date | Jun 22, 1976 |
| Priority date | — |
| Expiry date | Jan 30, 1995 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A preweighed vial of lyophilized methemoglobin (ferrihemoglobin) provides a simple, stable, reproducible standard for colorimetric determination of total hemoglobin concentration in whole blood by the cyanmethemoglobin method. Conversion of the standard and of hemoglobin in blood samples to cyanmethemoglobin is accomplished with Drabkin's solution reconstituted from a dry powder and having an added non-ionic surface active agent (Brij-35). Color intensity is measured at 540 m.mu..
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