Enzymatic determination of glucose

US3964974A · kind A · utility

Assignee

• Merck Patent GmbH · DE

Inventors

• Dieter Banauch
• Wolfgang Brummer
• Wolfgang Ebeling · Hannover, DE
• Roland Helger · Darmstadt, DE
• Norbert Hennrich
• Hermann Lang

Key dates

• Filing date: Sep 10, 1973
• Grant date: Jun 22, 1976
• Expiry date: Sep 10, 1993

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/54
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Glucose is rapidly ad quantitatively determined with an analytical agent containing glucose dehydrogenase having an activity of at least 2 .mu./mg and NADH.sub.2 oxidase activity less than 0.1%, a pyridine coenzyme, a buffer and mutarotase to increase spontaneous mutarotation of alpha-glucose to beta-glucose. There may be optionally present inhibitors for reduced pyridine coenzyme oxidases and a thermal stabilizing amount of alkali metal chloride.