Vertical gel slab electrophoresis apparatus and method
US3980540A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 18, 1975 |
| Grant date | Sep 14, 1976 |
| Priority date | — |
| Expiry date | Aug 18, 1995 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44756
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A gel electrophoresis device in which the separating gel is cast in a vertical position between hydrophilic plates which form a gel slab chamber filled with a separating gel solution from the bottom for reducing fill turbulence allowing formation of a gel slab having a predetermined gradient. A removable seal is applied to the bottom of the gel slab chamber for containing the separating gel solution prior to polymerization. One gel slab chamber may be removably attached to each vertical side of a central temperature control chamber which is completely enclosed and which has an inlet and an outlet channel for circulation of temperature control fluid therethrough. An upper buffer chamber which opens upwardly is formed above the central temperature control chamber in communication with the top of the vertical gel slab chambers. The top of the gel slab is formed using a well forming implement made of polymerization inhibiting material so that the implement may be withdrawn after polymerization without altering the desired shape of the top of the vertical gel slab. The gel slab chambers attached to the central enclosed temperature control and upper buffer chamber are lowered into an out…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.