Integral element for the analysis of cholesterol
US3983005A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1975 |
| Grant date | Sep 28, 1976 |
| Priority date | — |
| Expiry date | Apr 7, 1995 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/918
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, a support. Cholesterol oxidase and a composition for the hydrolysis of cholesterol esters comprising lipase having cholesterol esterase activity and protease are included in the element such that cholesterol esters contained in a sample applied to the spreading layer are saponified to free cholesterol and free cholesterol is decomposed in the presence of cholesterol oxidase to produce a detectable change related to the total cholesterol content of the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.