.gamma.-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING .gamma.-GLUTAMYL TRANSPEPTIDASE

US3986931A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Erich Bernt · Munich, DE
• Wolfgang Gruber · Bischofswerda, DE
• Erich Haid · Tutzing, DE
• Fritz Stahler
• August W. Wahlefeld · Hohenpeißenberg, DE
• Gunter Weimann

Key dates

• Filing date: Aug 4, 1975
• Grant date: Oct 19, 1976
• Expiry date: Aug 4, 1995

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/48
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

.gamma.-Glutamyl-4-nitroanilide compounds of the formula ##SPC1## Wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyltranspeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycyl-glycine and determining the liberation of the resulting p-nitroaniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.