Purification of hepatitis B surface antigen
US3994870A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 31, 1974 |
| Grant date | Nov 30, 1976 |
| Priority date | — |
| Expiry date | Jan 31, 1994 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/826
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
After a polyethylene glycol purification procedure, virus particles, viral components and virus associated particles having concanavalin A binding sites are further purified by subjecting such particles or components to affinity chromatography utilizing insoluble concanavalin A as a chromatography adsorbent and an eluant capable of interacting with concanavalin A to thereby inhibit the binding of the virus particles or components to the insoluble concanavalin A. Specifically, the insoluble concanavalin A may be concanavalin A linked to agarose beads and the eluant may be methyl-.alpha.-D-mannopyranoside. Specifically, these procedures may be utilized to purify hepatitis B antigen (HB Ag) particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.