Method of discriminating between dyed particles and background fluorescence of the dye
US4006360A · kind A · utility
60Cited by
2References
8Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jan 2, 1976 |
| Grant date | Feb 1, 1977 |
| Priority date | — |
| Expiry date | Jan 2, 1996 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6408
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system for discriminating between the fluorescent emission from dye molecules bound to biological particles and the background emission from free molecules of the dye in the solution suspending the particles, where the quantum efficiency of the bound dye molecules because of their bound state, differs from the quantum efficiency of the unbound dye so that the emission from the bound dye exhibits a longer statistical decay lifetime. The system involves irradiating the particles in the solution with a mode locked laser, and measuring fluorescent intensity in delayed synchronism with the mode locking frequency.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.