Method for preparing a heat-stable plasma protein solution from paste IV-1
US4017470A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 12, 1974 |
| Grant date | Apr 12, 1977 |
| Priority date | — |
| Expiry date | Jun 12, 1994 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/831
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A heat-stable plasma protein solution showing no blood pressure-depressing action is obtained by adding distilled water to a paste IV-1, a waste fraction obtained from the Cohn's cold ethanol plasma fractionation method and has heretofore been discarded as a waste, to extract water-soluble proteins in the paste, heat-treating the extract at pH 4.5 to 5.5 at 50.degree. to 65.degree. C. in the presence of an organic acid to remove as precipitates lipo- and glyco-proteins contained in the extract, adding Rivanol to the resulting supernatant to remove by precipitation residual lipo-proteins giving turbidity to the supernatant, and then removing blood pressure-depressing substances present in the resulting supernatant by adsorption with an inorganic adsorbent or cation exchanger. The thus obtained heat-stable plasma protein solution shows prominent effects in the therapy of shock by acute bleeding, burns, supply of protein nutrients, hypoproteinemia, etc., and is useful for medical treatments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.