Method for quantitation of antigens or antibodies on a solid surface
US4020151A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 17, 1976 |
| Grant date | Apr 26, 1977 |
| Priority date | — |
| Expiry date | Feb 17, 1996 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/825
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for quantitation of antigens or antibodies from a liquid sample on a solid surface. The sample is first sorbed directly to a nonimmunological surface. Then the surface is exposed to antibody or antigen labelled preferably with a fluorogen, to cause an immunological reaction to occur. After removal of unreacted reagent from the surface, the quantity of reacted labelled reagent is determined, as for example, fluorogen by a fluorometer. When the sample includes significant quantities of protein other than the antigen or antibody to be quantitated, buffer protein unreactive with the labelled antigen or antibody is added in concentrations sufficient to minimize the dependence of sorbed antigen or antibody upon variations in the concentration of such other protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.