Isolation of coagulation factors I and VIII from biological material

US4022758A · kind A · utility

Assignee

• AB Kabi · SE

Inventors

• Lars Andersson · Knivsta, SE
• Håkan Borg · Wasserburg am Inn, DE
• Nanna Forsman · Järfälla, SE
• Gunnar Hanshoff · Stockholm, SE
• Goran Lindroos
• Maggie Miller-Andersson
• Elisabeth Charlotte Carling nee Ehrenberg

Key dates

• Filing date: Jul 30, 1975
• Grant date: May 10, 1977
• Expiry date: Jul 30, 1995

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/831
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The blood coagulation factors I (fibrinogen) and VIII (antihemophilia factor, abbreviated AHF) are isolated in high yields from animal tissue materials such as blood or blood products (e.g. plasma) or plasma fractions by a procedure involving the essential step of adsorption (as in affinity chromatography) of at least one of these factors, in a liquid system, onto a water-insoluble gel matrix primarily composed of a cross-linked sulfated or sulfonated, gel-forming carbohydrate such as cross-linked dextran sulfate-agarose, cross-linked dextran sulfate-dextran, cross-linked heparin-agarose and other such gel matrix-providing substances.