Protein-assay reagent and method
US4023933A · kind A · utility
22Cited by
4References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 10, 1976 |
| Grant date | May 17, 1977 |
| Priority date | — |
| Expiry date | Jun 10, 1996 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6839
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A dye binding reagent for protein assay comprises Coomassie Brilliant Blue G-250 and an acid having a pKa of from 1 to 2. Upon addition of the reagent to a protein-containing solution, the attendant color change can be spectrophotometrically measured to quantitate even micrograms of protein. Furthermore, the reagent is applicable to a wide range of proteins and requires only about 2 minutes for measurement. Addition of an alcohol such as ethanol to the reagent further improves sensitivity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.