Enzyme electrode

US4024042A · kind A · utility

Assignee

• Servochem AB

Inventors

• Sven-Olof Enfors · Sollentuna, SE
• Nils Molin
• Klaus Mosbach · Halmstad, SE
• Hans Nilsson · Lund, SE

Key dates

• Filing date: Mar 17, 1976
• Grant date: May 17, 1977
• Expiry date: Mar 17, 1996

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/817
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to an enzyme electrode which can be sterilized and regenerated and which has a sensitive surface and an enzyme in contact therewith. A semipermeable membrane is arranged at a distance from the sensitive surface of the detecting electrode and defines a chamber containing the enzyme which is supplied to and discharged from the chamber through a supply conduit and a discharge conduit, respectively. A sterilization device for the enzyme is arranged in the supply conduit for the enzyme. The enzyme electrode is produced by means of the detecting electrode being sterilized in a manner known per se and by means of the enzyme being led into a chamber via a sterilization device through a supply conduit for contact with the sensitive surface of the detecting electrode and for contact with the semipermeable membrane arranged at a distance from said sensitive surface. The enzyme is continuously or intermittently supplied through the supply conduit simultaneously as previously supplied enzyme is discharged from the chamber through the discharge conduit.