Method of high speed scintillation autoradiography
US4035155A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 30, 1975 |
| Grant date | Jul 12, 1977 |
| Priority date | — |
| Expiry date | Oct 30, 1995 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01T1/2942
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Autoradiography using high specific activity tritiated thymidine (40-60 curies/millimole) and nuclear track emulsion impregnated with a liquid scintillator provides a very rapid method to determine labelling index and growth fraction of cell suspensions. Low temperature (-20.degree. to -85.degree. C) emulsion exposure for 20 - 60 minutes is sufficient for excellent labelling, thus allowing rapid clinical decision making. Exposure times for research techniques requiring very low energy labelling can be considerably shortened, e.g., from 6 months to 2 weeks or less. This technique therefore has broad applicability to biology and medicine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.