Polyiodothyronine immunoassay
US4043872A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 29, 1975 |
| Grant date | Aug 23, 1977 |
| Priority date | — |
| Expiry date | Dec 29, 1995 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/964
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method and enzyme conjugates are provided for determining physiological amounts of polyiodothyronine in physiological fluid, particularly serum. A homogeneous enzyme immunoassay is provided employing an enzyme-bound-polyiodothyronine having less than about 50 percent of the original enzyme activity, whose activity increases upon binding with a receptor. The enzyme-bound-polyiodothyronine, the unknown suspected to containing a polyiodothyronine, and receptor for the polyiodothyronine are combined in an aqueous medium containing substrate for said enzyme and the enzymatic activity of the solution determined. By comparing the result obtained with results obtained from standards having known amounts of polyiodothyronine, the amount of polyiodothyroninein the unknown can be determined. The enzyme-bound-polyiodothyronine employs an enzyme which is reversibly deactivated by polyiodothyronine, so as to be reactived by receptor binding to the polyiodothyronine. Particular enzymes include malate dehydrogenase and triose phosphate isomerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.