Biologically active substance, bestatin, and production thereof

US4052449A · kind A · utility

Assignee

• Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai · JP

Inventors

• Hamao Umezawa · Tokyo, JP
• Takaaki Aoyagi
• Tomio Takeuchi · Tokyo, JP
• Masa Hamada · Hoya, DE
• Yoshiro Okami · Tokyo, JP

Key dates

• Filing date: May 27, 1976
• Grant date: Oct 4, 1977
• Expiry date: May 27, 1996

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a new chemical named bestatin which inhibits aminopeptidase B, leucine aminopeptidase and bleomycin hydrolase, enhances the antitumor effect of bleomycin, has the chemical name [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl]-L-leucine and has the following structure: ##STR1## and also provides a process for the production thereof which comprises cultivating a strain of streptomyces which produces bestatin in a nutrient medium under aerobic conditions until substantial activity inhibitory to aminopeptidase B is imparted to said cultured medium and then recovering said bestatin from said cultured medium. A preferred strain is Streptomyces olivoreticuli FERM-P No. 2590 (A.T.C.C. 31159).