Process for storing and recovering plant tissue
US4052817A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 12, 1976 |
| Grant date | Oct 11, 1977 |
| Priority date | — |
| Expiry date | Oct 12, 1996 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H4/00
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
Meristematic tissue such as from shoot apices of herbaceous plants in a freezing solution containing a cryoprotectant are cooled to a temperature of at least -70.degree. C, stored when frozen and subsequently thawed at an average warming rate of at least about 24.degree. C/min when the tissue is at a temperature between about -70.degree. C and about -10.degree. C to obtain pathogen-free meristematic tissue having morphogenic potential. The donor plants from which the tissue is to be excised are cold treated to induce cellular reactions believed to be related to the winter cold hardening process, generally at a temperature between the plant freezing temperature and about 10.degree. C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.