Adaptation of cell lines to suspension culture
US4059485A · kind A · utility
19Cited by
1References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 3, 1976 |
| Grant date | Nov 22, 1977 |
| Priority date | — |
| Expiry date | Nov 3, 1996 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2509/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Densely packed cells growing rapidly in substrate-attached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 .mu. to 500 .mu. in diameter. These aggregates are then transferred to fresh medium in agitated liquid suspension. The cells thereby continue to grow in aggregates whereby a normally anchorage-dependent estabished mammalian cell line is rapidly adapted to suspension culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.