Peptide or protein sequencing method and apparatus
US4065412A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 7, 1976 |
| Grant date | Dec 27, 1977 |
| Priority date | — |
| Expiry date | May 7, 1996 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/14
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method and apparatus for the sequential degradation of protein or peptide molecules by successive coupling and cleavage reactions. Such molecules are immobilized on a macroporous reaction support surface and placed in a flowthrough reaction chamber which is mounted in a sequencer. In the Edman sequencing technique, reagents driven by pressurized inert gas are passed through the reaction chamber as follows: (a) liquid or vapor coupling reagent, (b) coupling base vapor, (c) washing solvent, (d) inert gas to partially dry the support surface, (e) cleavage reagent vapor to cleave amino acid derivatives from the immobilized coupled protein or peptide chains, (f) liquid extracting solvent to withdraw the cleaved amino acid derivative. An automated sequencer to carry out said method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.