Patent · US Expired

Chromogen-reactive-indicator preparations containing A 3,3'-di(carbonyloxy- or sulfonyloxy-group-containing) benzidine derivative chromogen

US4066408A · kind A · utility

4Cited by
8References
18Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 9, 1977
Grant dateJan 3, 1978
Priority date
Expiry dateFeb 9, 1997

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2326/10
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

An analytical test, color change reagent preparation comprising peroxidase, an oxygen-oxidoreductase specific to a particular substance to be tested and a chromogen-reactive-indicator formula selected from (a) those having the general formula ##STR1## wherein X is the divalent carbonyloxy or sulfonyloxy group, W is hydrogen or another cation compatible with the aforesaid other ingredients of said reagent preparation, the subscript n is 0 or 1, and (i) when X is the carbonyloxy group, A is a divalent alkylene chain with from 2 to about 7 carbons, and (ii) when X is the sulfonyloxy group, n is 0 when A is the divalent alkylene chain of from 2 to about 7 carbons, and n is 1 when A is straight chain trimethylene or tetramethylene, and (b) the acid addition salts of said disubstituted benzidine derivative, compatible with the aforesaid ingredients of the chromogen-reactive-indicator, when X is the carbonyloxy group. The reagent preparations may be used in assaying organic substances such as sugars, uric acid, an amino acid and pyridoxaminophosphate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.