Colorimetric assay for bilirubin
US4069017A · kind A · utility
71Cited by
5References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 14, 1977 |
| Grant date | Jan 17, 1978 |
| Priority date | — |
| Expiry date | Jan 14, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/146666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A colorimetric assay method for the detection of bilirubin in various liquid samples, particularly biological liquids. A multi-zone analytical element based on this assay method is also disclosed. The assay method employs an interactive mordant composition for bilirubin having one or more binding sites for bilirubin, such composition comprising a hydrophobic organic matrix and at least one charge-bearing cationic group. Upon mordanting of bilirubin by the interactive mordant composition, the mordanted bilirubin exhibits an absorption peak shift of at least 10 nm. and at least a 50% increase in molar extinction coefficient.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.