Production of viruses in tissue culture without use of serum
US4072565A · kind A · utility
35Cited by
0References
15Claims
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Assignee
Inventors
Key dates
| Filing date | Nov 4, 1974 |
| Grant date | Feb 7, 1978 |
| Priority date | — |
| Expiry date | Nov 4, 1994 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/36251
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Growth of cells in tissue culture and virus vaccine production in tissue culture in the absence of serum are disclosed. The cells in tissue culture are grown initially in a chemically defined medium containing protamine zinc insulin, and the protamine zinc insulin medium is removed before virus propagation. Supplementation with serum or serum fractions can be eliminated entirely; and improved virus production in a serum free system can be obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.