System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents
US4072574A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 30, 1976 |
| Grant date | Feb 7, 1978 |
| Priority date | — |
| Expiry date | Jul 30, 1996 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/48
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This involves an in vitro assay system to screen for mutagenic and/or carcinogenic agents based on alterations in the fidelity of DNA synthesis. In the system, chemicals will be tested for their mutagenicity or carcinogenicity by measuring increases in the number of mistakes incorporated by purified DNA polymerases using synthetic polynucleotide templates. This system offers the advantage of performing this analysis in the test tube so that all parameters in the reaction can be monitored. Agents that enhance misincorporation during DNA synthesis would be predicted to have a higher probability of being mutagens or carcinogens in the human population.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.