Patent · US Expired

Polypeptide hormones of the thymus

US4077949A · kind A · utility

25Cited by
0References
8Claims
0Family size

Assignee

Inventor

Key dates

Filing dateAug 22, 1975
Grant dateMar 7, 1978
Priority date
Expiry dateAug 22, 1995

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S930/18
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

There are disclosed closely related new polypeptides isolated from bovine thymus, which are distinct polypeptides denoted as Thymin I and Thymin II or Thymopoietin I and Thymopoietin II, respectively. Thymin I may be characterized as having a relative mobility (Rf) on 7% polyacrylamide gel disc electrophoresis of 0.63 with respect to methyl green at a pH of 4.3, and having the following structural sequence: PA1 H.sub.2 n-gly-gln-phe-leu-glu-asp-pro-ser-val-leu-thr-lys-glu-lys-leu-lys-ser-glu- leu-val-ala-asn-asn-val-thr-leu-pro-ala-gly-glu-gln-arg-lys-asp-val-tyr-val -gln-leu-tyr-leu-gln-his-leu-thr-ala-val-lys-arg-cooh. Thymin II may be characterized as having a relative mobility (Rf) on 7% polyacrylamide gel disc electrophoresis of 0.57 with respect to methyl green at a pH of 4.3, and having the following structural sequence: PA1 H.sub.2 n-ser-gln-phe-leu-glu-asp-pro-ser-val-leu-thr-lys-glu-lys-leu-lys-ser-glu- leu-val-ala-asn-val-thr-leu-pro-ala-gly-glu-gln-arg-lys-asp-val-tyr-val-gln -leu-tyr-leu-gln-thr-leu-thr-ala-val-lys-arg-cooh. Both polypeptides are highly purified and in powder form and are isolated from bovine thymus by multiple purification methods involving molecular …

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