Process and reagent for the rapid quantitative determination of lactate or alanine

US4080263A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Erich Bernt · Munich, DE
• Peter Scheibe · Munich, DE
• Ingeborg Gutmann · Penzberg, DE

Key dates

• Filing date: Aug 11, 1976
• Grant date: Mar 21, 1978
• Expiry date: Aug 11, 1996

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/32
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

L-lactate is quantitatively determined by the reaction thereof with nicotinamide-adenine-dinucleotide (NAD) in the presence of lactate dehydrogenase (LDH) with the formation of reduced NAD (NADH) and pyruvate, reaction of the latter with glutamate in the presence of glutamate-pyruvate-transaminase (GPT) with the formation of alanine and .alpha.-ketoglutarate in alkaline buffered solution and measurement of the NADH formed, wherein the reaction is carried out in the presence of at least 0.4 mol/liter of carbonate or bicarbonate.