Preparation of carrier-bound macromolecular compounds

US4081329A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Dieter Jaworek
• Karl-Heinz Botsch
• Gunter Weimann
• Michael Nelbock-Hochstetter · Tutzing, DE
• Helmut Determann

Key dates

• Filing date: Feb 5, 1976
• Grant date: Mar 28, 1978
• Expiry date: Feb 5, 1996

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/813
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Carrier-bound macromolecular compounds, especially bioactive macromolecular compounds, are produced by reacting a macromolecular compound A with a coupling compound B containing at least one reactive function capable of coupling with compound A and at least one further reactive function capable of polymerization, adding to the resulting coupled reaction product AB an unswollen molecular sieve material having cross-linking sufficient to exclude penetration by compound A, and polymerizing the polymerizable group of the coupled product AB in the molecular sieve material to form a polymer body in the molecular sieve material and fix compound A on the surface of the molecular sieve material. Compound A can be, e.g., an enzyme, antibody or hormone, and coupling compound B typically contains, as the coupable function, an alkylation or acylation group such as oxirane, ethyleneimine, halide, acid halide, azide or anhydride groups, and, as the polymerizable function, a carbon-carbon or carbon-oxygen double bond.