Blood fractionation

US4081431A · kind A · utility

Assignee

• Biotest-Serum-Institut GmbH

Inventors

• Wolfgang Stephan · Heidelberg, DE
• Ronald Kotitschke · Dreieich, DE

Key dates

• Filing date: Dec 11, 1975
• Grant date: Mar 28, 1978
• Expiry date: Dec 11, 1995

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/83
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A process for the fractionation of blood comprising passing said blood through a cation exchanger, separating the solids from the plasma, freezing the plasma, thawing the frozen plasma, and separating a first product comprising undissolved cryoprecipitate enriched in factor-VIII protein from plasma fluid. The cryoprecipitate can be concentrated by warm water dissolution and polyethylene glycol precipitation. The plasma fluid, optionally after treatment with .beta.-propiolactone and uv irridiation, is treated with tricalcium phosphate to adsorb factors II, VII, IX and X as a second product. These factors can be eluted with citrate solution. The residual plasma from the initial tricalcium phosphate adsorption is treated with colloidal silica to adsorb impurities and leave a third product which is a storage-stable serum protein solution.