Process for the production of insulin by genetically transformed fungal cells
US4082613A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 23, 1976 |
| Grant date | Apr 4, 1978 |
| Priority date | — |
| Expiry date | Apr 23, 1996 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the production of insulin using fungal cells. Human insulin producing cells are extracted to obtain the functional genome (the genetic material which determines the capacity of the cells to produce insulin) which is used to transform fungal cells, making them capable of producing insulin. The insulin has been extracted and identified by radioimmune assay and by bioassay. The same process can also be applied to produce animal insulins using specific specie transforming functional insulinogenic genomes. A process for serial secondary culture of insulin producing cells is disclosed. The significance of the invention is related to the great need to produce human insulin in large amounts. Human insulin has distinct biological and clinical advantages when compared with commercially available animal insulins now used for the treatment of diabetes mellitus in man.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.