Colorimetric method for determining gamma-glutamyl transpeptidase and compositions useful therein
US4087331A · kind A · utility
11Cited by
2References
9Claims
0Family size
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Key dates
| Filing date | Jan 12, 1977 |
| Grant date | May 2, 1978 |
| Priority date | — |
| Expiry date | Jan 12, 1997 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/48
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A colorimetric method for the determination of gamma-glutamyl transpeptidase enzyme activity in a biological fluid comprises catalytically reacting the enzyme in the fluid with a substrate of a water-soluble salt of a compound selected from the group consisting of L-gamma-glutamyl-3-sulfoanilide and L-gamma-glutamyl-4-sulfoanilide in aqueous solution to produce the corresponding aniline sulfonic acid salt as a cleavage product, diazotizing the cleavage product, and coupling the diazotized product with a coupling compound to produce a dye having a color intensity proportional to the enzyme activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.