Reverse sandwich immunoassay
US4098876A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 26, 1976 |
| Grant date | Jul 4, 1978 |
| Priority date | — |
| Expiry date | Oct 26, 1996 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method of determining the presence and/or concentration of a polyvalent antigenic substance in a fluid. The method comprises the steps of incubating the fluid with labeled antibodies to the substance to form a first labeled immunochemical complex and then incubating that complex with immobilized antibodies to the substance to form a second labeled complex which is separated from the incubation medium. The amount of label in the second complex provides a means for detecting and/or quantitating the substance in the fluid. By reversing the sequence of incubation steps in a "two-site" or sandwich assay, greater sensitivity is achieved and an intermediate washing step is eliminated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.