Patent · US Expired

Method and reagent for the determination of substances forming hydrogen peroxide

US4101381A · kind A · utility

19Cited by

2References

14Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Sigmar Klose · Starnberg, DE
Ulfert Deneke · Fürth, DE
Erich Haid · Tutzing, DE
Gunter Weimann

Key dates

Filing date	Dec 27, 1976
Grant date	Jul 18, 1978
Priority date	—
Expiry date	Dec 27, 1996

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2326/00
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

In a conventional method for the quantitative determination of substances, particularly in serum, which, by action of an oxidase thereon, combine with oxygen to form hydrogen peroxide and wherein the hydrogen peroxide formed is converted, in the presence of methanol and catalase, to formaldehyde, the formaldehyde is reacted with hydrazone in the presence of ferric chloride, and the dye thus formed is determined photometrically, the improvement comprising using as the hydrazone, 3-methyl-2-sulfonylbenzothiazolone-hydrazone; use of this hydrazone results in the formation of a very easily soluble dye and is suited for use in automatic analyzers.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.