Fluid isolation electrophoresis apparatus and method
US4101401A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 11, 1977 |
| Grant date | Jul 18, 1978 |
| Priority date | — |
| Expiry date | Mar 11, 1997 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44704
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Electrophoretic separation of samples in a running gel is obtained by depositing three layers of liquid in an outer container. The liquid layers have increasing specific gravities proceding from the uppermost to the lowermost layer. The upper and lower layers are electrically conductive buffer solutions which are immiscible with the center layer which is electrically insulating. A rack is provided for holding running gels in the outer container so that the upper end of the gels are in contact with the upper buffer layer and the lower end of the gels are in contact with the lower buffer layer. An upper and a lower electrode is provided in contact with each of the upper and lower buffer solutions respectively. A voltage impressed between the upper and lower electrodes provides a substantially unidirectional electrical field through the running gel and separation of samples in the running gel substantially free of distortion due to the absence of spurious electrical fields at the sides of the running gel adjacent to the central layer of electrically insulating fluid. Removal of the heat of electrophoresis and further reduction of distortion in separated samples is achieved by cooling …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.